Solid lipid nanoparticles loading adefovir dipivoxil for antiviral therapy.
- Author:
Xing-guo ZHANG
1
;
Jing MIAO
;
Min-wei LI
;
Sai-ping JIANG
;
Fu-qiang HU
;
Yong-zhong DU
Author Information
- Publication Type:Journal Article
- MeSH: Adenine; administration & dosage; analogs & derivatives; pharmacokinetics; Amines; Antiviral Agents; administration & dosage; pharmacokinetics; Cell Line; Drug Delivery Systems; Fluorescein-5-isothiocyanate; Fluorescent Dyes; Glycerides; Hepatitis B virus; drug effects; Humans; Nanoparticles; Nanotechnology; Organophosphonates; administration & dosage; pharmacokinetics
- From: Journal of Zhejiang University. Science. B 2008;9(6):506-510
- CountryChina
- Language:English
- Abstract: Herein, solid lipid nanoparticles (SLN) were proposed as a new drug delivery system for adefovir dipivoxil (ADV). The octadecylamine-fluorescein isothiocynate (ODA-FITC) was synthesized and used as a fluorescence maker to be incorporated into SLN to investigate the time-dependent cellular uptake of SLN by HepG2.2.15. The SLN of monostearin with ODA-FITC or ADV were prepared by solvent diffusion method in an aqueous system. About 15 wt% drug entrapment efficiency (EE) and 3 wt% drug loading (DL) could be reached in SLN loading ADV. Comparing with free ADV, the inhibitory effects of ADV loaded in SLN on hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg) and hepatitis B virus (HBV) DNA levels in vitro were significantly enhanced.
