Arylamine N-acetyltransferases: a new inhibitor of apoptosis in HepG2 cells.

Author: Yu-bin JI ¹ ; Shi-yong GAO
Author Information

1. Postdoctoral Research Station of the Institute of Materia Medica, Center for Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China.
Publication Type:Journal Article
MeSH: Apoptosis; drug effects; Arylamine N-Acetyltransferase; antagonists & inhibitors; metabolism; Camptothecin; administration & dosage; Cells, Cultured; Dose-Response Relationship, Drug; Hepatocytes; cytology; drug effects; enzymology; Humans
From: Journal of Zhejiang University. Science. B 2008;9(9):701-706
CountryChina
Language:English
Abstract:
OBJECTIVETo explore how arylamine N-acetyltransferases (NATs) is related to cell apoptosis.
METHODSNAT activity in apoptotic HepG2 cells was measured using high performance liquid chromatography (HPLC); the apoptosis rate of HepG2 cells acted upon by an NAT inhibitor was measured using flow cytometry.
RESULTSNAT activity was lowered in apoptotic HepG2 cells; apoptosis rate induced by camptothecin (CAM) increased after inhibition of NAT activity in HepG2 cells.
CONCLUSIONNAT can inhibit apoptosis in HepG2 cells.