Effect of acupuncture on serum MIP-2 and MIP-2 mRNA expressions in isolated Fei and Dachang of severe acute pancreatitis induced acute lung injury rats in the acute phase.
- Author:
Li-Ya JIANG
1
;
Ji-Ren HUANG
;
Hong-Qing ZHAO
;
Jing-Fen ZHU
;
Jian-Liang DAI
;
Wei-Dong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Acupuncture Therapy; Acute Lung Injury; blood; complications; metabolism; Animals; Chemokine CXCL2; blood; genetics; metabolism; Disease Models, Animal; Intestine, Large; metabolism; Lung; metabolism; Male; Pancreatitis; blood; complications; metabolism; RNA, Messenger; genetics; Rats; Rats, Wistar
- From: Chinese Journal of Integrated Traditional and Western Medicine 2013;33(7):958-962
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe effect of acupuncture on serum macrophage inflammatory protein-2 (MIP-2) and MIP-2 mRNA expressions in isolated Fei and Dachang of severe acute pancreatitis (SAP) induced acute lung injury (ALI) rats in the acute phase.
METHODSForty male Wistar rats were randomly divided into four groups, i.e., the sham-operation group, the SAP group, the acupuncture treatment group, and the acupuncture control group, 10 in each group. The SAP model was induced by retrograde infusion of 3.5% sodium taurocholate into the pancreatobiliary duct. Under the guidance of "Fei and Dachang exterior-inferiorly related", points were acupunctured along Fei, Dachang, and Pi channels, as well as those points on the back of rats in the acupuncture treatment group 0.5 h after modeling. Besides, points were acupunctured along Fei and Pi channels, as well as those points on the back of rats in the acupuncture control group 0.5 h after modeling. Serum levels of tumor necrosis factor alpha (TNF-alpha) and nitric oxide (NO), and MIP-2 expressions were examined 6 h after modeling. Expressions of MIP-2 mRNA in isolated lung and large intestine tissues were detected by reverse transcription PCR.
RESULTSCompared with the sham-operation group, serum levels of TNF-alpha and NO, and expressions of MIP-2 and MIP-2 mRNA in isolated lung and large intestine tissues were significantly higher in the SAP group (P < 0.05). Each index was lower in the acupuncture treatment group than in the SAP group and the acupuncture control group (P < 0.05). Besides, the serum level of MIP-2 and the MIP-2 mRNA expression in isolated lung and large intestine tissues were positively correlated in all groups except the sham-operation group (P < 0.05).
CONCLUSIONSUnder the guidance of "Fei and Dachang exterior-inferiorly related", acupuncture could remarkably reduce the severity of SAP induced ALI rats in the acute phase. Its mechanism might be related to suppressing over-expressions of MIP-2 mRNA in isolated lung and large intestine tissues, and lowering the serum MIP-2 expression level.
