Xuefu zhuyu decoction containing serum in vitro induced expressions of desmin and alpha-actin: an experimental research.
- Author:
Yue-Xiang MA
1
;
Li-Qing LIU
;
Li-Mei QIN
;
Guang-Min ZUO
;
Yuan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Actins; metabolism; Animals; Bone Marrow Cells; cytology; drug effects; metabolism; Cells, Cultured; Desmin; metabolism; Drugs, Chinese Herbal; pharmacology; Male; Mesenchymal Stromal Cells; cytology; drug effects; metabolism; Rats; Rats, Wistar; Serum
- From: Chinese Journal of Integrated Traditional and Western Medicine 2013;33(9):1252-1255
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe whether Xuefu Zhuyu Decoction (XZD) could induce the differentiation of mesenchymal stem cells (MSCs) into cardiac myoid cells, thus seeking for safe and effective inducers.
METHODSThe serum pharmacological method was used to induce. XZD containing serum was prepared. MSCs were isolated and cultured. The serum cytotoxicity was detected by MTT. The third generation of favorably grown cells was selected in this experiment. Cells were divided into three groups, i.e., the vehicle control group, the XZD containing serum induced group, and the 5-azacytidine induced group. Expressions of Desmin and alpha-actin were detected by immunocytochemical staining method.
RESULTSBefore induction protein expressions of Desmin and alpha-actin were negative, and few was weakly positive. There was no statistical difference in the weak positive expression rate among the 3 groups (P > 0.05). After induction protein expressions of Desmin and alpha-actin were negative, and few was weakly positive in the vehicle control group. Protein expressions of Desmin and alpha-actin were positive in the XZC containing serum induced group and the 5-azacytidine induced group. There was statistical difference in the positive expression rate when compared with the vehicle control group (P > 0.05).
CONCLUSIONSXZD played a role in in vitro inducing differentiation MSCs to cardiac myoid cells. It might participate in expressions of Desmin and alpha-actin.