Radiosensitization effect of black garlic extract on lung cancer cell line Lewis cells.

Gui-qing Yang; Dong Wang; Yi-shan Wang; Yuan-yuan Wang; Ke Yang

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Radiosensitization effect of black garlic extract on lung cancer cell line Lewis cells.

Author: Gui-qing YANG ¹ ; Dong WANG ; Yi-shan WANG ; Yuan-yuan WANG ; Ke YANG
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1. National Key Department of Tumor Treatment by Integrative Medicine/Non-traumatic Center for Tumor Diagnosis and Treatment of the Whole Army, 107th Hospital of the People's Liberation Army, Shandong (264002), China.
Publication Type:Journal Article
MeSH: Animals; Apoptosis; Carcinoma, Lewis Lung; metabolism; pathology; Cell Line, Tumor; drug effects; radiation effects; Garlic; Mice; Plant Extracts; pharmacology; Proto-Oncogene Proteins c-bcl-2; metabolism; Radiation-Sensitizing Agents; pharmacology; bcl-2-Associated X Protein; metabolism
From: Chinese Journal of Integrated Traditional and Western Medicine 2013;33(8):1093-1097
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo explore the radiosensitization effect of black garlic extract (BGE) on lung cancer cell line Lewis cells.
METHODSThe inhibition rate of lung cancer cells after BGE action was detected by MTT. Effect of BGE combined radiotherapy on the colony formation rate was observed by cloning formation assay. Changes of the cell morphology were observed by Hoechst staining. Changes of the cell cycle were detected by flow cytometry. Real time PCR was used to detect mRNA expressions of bcl-2 and bax.
RESULTSBGE could have significant inhibitory action on the growth of lung cancer Lewis cells. The combination of BGE and radiotherapy (by 60Co gamma) significantly induced Lewis cells' apoptosis in G2/M stage, obviously decreased the expression of bcl-2, and up-regulated the expression of bax.
CONCLUSIONSBGE could sensitize the lung cancer Lewis cells to ionizing irradiation. This effect might be probably caused by changing the cell cycles and affecting expressions of bax and bcl-2.