Protection against acute hypoxic/reoxygenation injury to kidney for rabbit with morphine hypoxic preconditioning by observing the expression of caspase-3 protein.
- Author:
Xiaoqin JIANG
1
;
Juan NI
;
Pei YANG
;
Wei HUANG
;
Jinfeng LUO
;
Huafeng LI
;
Yaping WANG
Author Information
1. Department of Anesthesiology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Caspase 3;
metabolism;
Ischemic Preconditioning;
methods;
Kidney;
blood supply;
metabolism;
Male;
Morphine;
pharmacology;
Rabbits;
Random Allocation;
Reperfusion Injury;
prevention & control;
Superoxide Dismutase;
metabolism
- From:
Journal of Biomedical Engineering
2011;28(3):531-533
- CountryChina
- Language:Chinese
-
Abstract:
The maintenance of the balance between oxygen supply and oxygen consumption is a key measure in preventing acute kidney hypoxic/reoxygenation injury. Morphine can inhibit metabolism and reduce the oxygen consumption. We tried to investigate the protective effects of morphine hypoxic preconditioning on acute kidney hypoxic/reoxygenation injury in rabbit and its influence on expression of caspase-3 protein. Kidney hypoxic and reoxygenation were induced by making the tested rabbits inhale 8% oxygen for three hours firstly, and then putting them in the air to breathe in normal oxygen for another three hours. Morphine hypoxic preconditioning was induced by administering morphine 3 mg/kg, and then hypoxic of 8% oxygen was induced. Caspase-3 protein expression in renal tissue was assessed by immunohistochemical method. In the present study, the expressions of caspase-3 protein were significantly higher in saline-control hypoxic group than in morphine hypoxic preconditioning group ((29.3+/-5.7)% vs. (12.16+1.23)%, P<0.05). These observations suggested that morphine hypoxic preconditioning can protect rabbit against acute kidney hypoxic/reoxygenation injury by decreasing expression of caspase-3 protein.
