Construction and expression of hTNF-alpha fusion protein mediated by MMP1.
- Author:
Qiaojiajie ZHAO
1
;
Gan HOU
;
Dinan HUANG
;
Shuyong CHEN
Author Information
1. Department of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang 524023, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Escherichia coli;
genetics;
metabolism;
Humans;
Matrix Metalloproteinase 1;
biosynthesis;
genetics;
Molecular Sequence Data;
Recombinant Fusion Proteins;
biosynthesis;
genetics;
Tumor Necrosis Factor-alpha;
biosynthesis;
genetics
- From:
Journal of Biomedical Engineering
2011;28(3):534-537
- CountryChina
- Language:Chinese
-
Abstract:
This paper is aimed to present a research on fusion protein of human tumor necrosis factor-alpha (hTNF-alpha), matrix metalloproteinase 1 (MMP1), and foldon sequence using the methord of gene engineering. We transformed the recombinant plasmid, which contains the DNA sequences of hTNF-alpha, MMP1, and foldon sequence, into Rosetta2, and successfully induced the fusion protein to express under given conditions by isopropyl beta-D-1-Thiogalactopyranoside (IPTG). Then we purified the expression product through a glutathione S-transferase (GST) resin and collected the interested protein. This research may lay the groundwork for scientific research and clinical application.
