Effects of the symbiosis of Trichomonas vaginalis with Mycoplasma hominis on ferredoxin gene.
- Author:
Xiaodong LIU
1
;
Wenjing WEN
;
Changgui XUE
Author Information
1. Department of Human Anatomy and Embryology, Luohe Medical College, Luohe 462002, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Base Sequence;
Female;
Ferredoxins;
genetics;
Humans;
Molecular Sequence Data;
Mycoplasma hominis;
genetics;
physiology;
Symbiosis;
genetics;
Trichomonas vaginalis;
genetics;
physiology
- From:
Journal of Biomedical Engineering
2011;28(4):800-803
- CountryChina
- Language:Chinese
-
Abstract:
We isolated 30 Trichomonas vaginalis for the PCR detection from the gynecological outpatients in the Affiliated Hospital of Zhengzhou University using the specific 16s rDNA primers of Mycoplasma hominis. The results showed that there were 25 cases of Mycoplasma hominis infection, with the infection rate of 83.33%. This gave a clew that the symbiosis of Trichomonas vaginalis with Mycoplasma hominis may be of certain generality in China. We sequenced the ferredoxin gene of 10 Trichomonas vaginalis where 5 Mycoplasma hominis were positive and five negative, and found that the ferredoxin (Fd) gene of the 10 Trichomonas vaginalis were exactly the same. But compared to the genes in the GenBank, a comparative analysis of the gene revealed that there were 3 more ctg bases at the 200th position of encoding leucine, but this did not lead to changes in reading frame. The gene homology was 99%.
