Preliminary research of induction of the multiple HPV antibody by HPV L1 type conserved sequence aimed at human papillomavirus major protein.
- Author:
Bao LIN
1
;
Xia WANG
;
Yi ZHANG
;
Henxi CHENG
;
Lina HU
Author Information
1. Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Viral;
immunology;
B-Lymphocytes;
immunology;
Capsid Proteins;
classification;
immunology;
Computer Simulation;
Epitopes;
immunology;
Female;
HeLa Cells;
Humans;
Oncogene Proteins, Viral;
classification;
immunology;
Papillomaviridae;
immunology;
Papillomavirus Infections;
immunology;
Rabbits;
Viral Proteins;
immunology
- From:
Journal of Biomedical Engineering
2011;28(5):982-987
- CountryChina
- Language:Chinese
-
Abstract:
To investigate whether a conserved sequence of the human papillomavirus(HPV) L1 protein consisted of 12 amino acid residue can induce the antibody aimed at multiple HPV types, we screened a conserved sequence of the HPV L1 protein by forecasting B cell epitope and comparing multiple sequences. The peptide was synthesized, mixed with Freund adjuvant, and used to immunize rabbits, and those in the control group were only immunized with Freund adjuvant. Then the antibody titer was identified by indirect enzyme-linked immunosorbent assay (ELISA). And immunocytochemistry, immunofluorescence, western blot and immunohistochemistry were used to detect whether the antibody could react with cervical cancer cell lines and cervical tissue that had been identified with HPV infections. We found that the antibody titer was greater than 1:25600. Moreover, we confirmed that the antibody could react with cervical cancer cell lines and cervical tissue with HPV infections. The results showed that the peptide could induce antibody aimed at multiple HPV types. Our findings have great significance in further research of the broad spectrum HPV, HPV L1 diagnosis kits.
