The Production of Phospholipase A2 in Different Types of Cultured Human Intervertebral Disc Cells.
- Author:
Dong Jun KIM
1
;
Jin Man WANG
Author Information
1. Department of Orthopaedic Surgery, Ewha Womans University Hospital, Seoul, Korea. Isthmic spondylolisthesis.
- Publication Type:Original Article
- Keywords:
Intervertebral disc;
Cell culture;
Lactate;
Phospholipase A2
- MeSH:
Blotting, Northern;
Cell Culture Techniques;
Humans*;
Intervertebral Disc*;
Lactic Acid;
Phospholipases A2*;
Phospholipases*
- From:Journal of Korean Society of Spine Surgery
2000;7(1):1-8
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
STUDY DESIGN: Evaluation of phospholipase A2 production according to cell type of human intervertebral disc. SUMMARY OF LITERATURE REVIEW: It was reported that the phospholipase A2 activity in human lumbar disc herniation was more active than that in other tissues. OBJECTIVES: The purpose of this study was to evaluate the differences between the cells of anulus fibrosus and nucleus pulposus when lactate was added to the culture medium. MATERIALS AND METHODS: Cells from the anulus fibrosus and nucleus pulposus of a human intervertebral disc were prepared enzymatically. After the monolayer was set up, the cells were divided to three groups and lactate doses of a 0mM, 2mM or 5mM were added respectively. At two week after lactate addition the production of phospholipase A2 was measured by Northern blotting. RESULTS: Cells of nucleus pulposus produced a small amount of phospholipase A2. Those of anulus fibrosus showed a high activity of phospholipase A2 production. The concentration of lactate did not influenced on the production of phospholipase A2. CONCLUSION: The anulus fibrosus has an important role in the production of phospholipase A2 and is thought to be related with generation of discogenic pain.
