Long non-coding RNA Gm15577 is involved in mouse cerebellar neurogenesis.
- Author:
Yongsong YUE
1
,
2
;
Weilong ZHANG
;
Chunying LIU
;
Yamei NIU
;
Weimin TONG
3
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; Cell Proliferation; Cell Transformation, Neoplastic; Cerebellar Neoplasms; pathology; Cerebellum; cytology; physiology; Disease Models, Animal; Humans; Introns; Medulloblastoma; pathology; Mice; Mice, Knockout; Neurogenesis; Neurons; physiology; RNA, Long Noncoding; metabolism
- From: Chinese Journal of Pathology 2015;44(7):504-508
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify novel lncRNAs involved in cerebellar neurogenesis using neuronal specific Nbs1-deficient (Nbs1(CNS-del)) mouse model.
METHODSMicroarray analysis was performed to identify differentially expressed lncRNAs between Nbs1(CNS-ctr) and Nbs1(CNS-del) mice. Expression profiles of lncRNA Gm15577 and coding gene Negr1 in mice, primary cerebellar culture and cell lines were measured using RT-qPCR. Subcellular fractionation was performed to determine the subcellular localization of Gm15577.
RESULTSGm15577 was specifically expressed in mice cerebellum in a developmentally regulated manner, which could be abolished upon Nbs1-deficiency. Gm15577 was located in the intronic region of Negr1 in a reversed orientation. Gm15577 modulated the RNA expression of Negr1, Shh and β-catenin. NEGR1 had a distinct expression pattern between normal and medulloblastoma patients.
CONCLUSIONGm15577 may modulate cerebellar granule cell proliferation and differentiation by targeting Negr1, and their dysfunctions or abnormal expression may be related to tumorigenesis of medulloblastoma.
