Mechanism of serotonin-promoted synthesis of osteoblast type I collagen.
- Author:
Yujing CAO
1
;
Cuitian LYU
2
;
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Cell Differentiation; Cells, Cultured; Collagen Type I; metabolism; Humans; Osteoblasts; drug effects; metabolism; Protein-Lysine 6-Oxidase; metabolism; RNA, Small Interfering; Serotonin; pharmacology; Signal Transduction; Smad2 Protein; metabolism; Smad3 Protein; metabolism; Up-Regulation
- From: Chinese Journal of Pathology 2015;44(7):509-512
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the mechanism of serotonin-promoted osteoblast differentiation.
METHODSExpression levels of collagen I and lysyl oxidase (LOX) in osteoblast were measured by RT-PCR after treated by (50, 100, 200 and 400 ng/L) serotonin. LOX siRNA effect was measured by Western blot, and protein levels of collagen I were determined by ELISA after treated by serotonin. Expression levels of Smad2 and Smad3 in osteoblasts were also measured by RT-PCR after treated by serotonin.Moreover, expression levels of LOX were measured by RT-PCR after Smad3 was knockout.
RESULTSSerotonin promoted collagen I and LOX expression. The expression level of collagen I was significantly decreased by LOX siRNA. Furthermore, serotonin up-regulated the expression of Smad2 and Smad3 in osteoblasts, and the expression level of LOX was inhibited by Smad3 siRNA.
CONCLUSIONSerotonin promoted collagen I expression by activating Smads signaling pathway and up-regulating the LOX expression.
