Effect of local administration of cyclosporine A on peripheral nerve regeneration in a rat sciatic nerve transection model.
- Author:
Rahim MOHAMMADI
1
;
Hadi HEYDARIAN
;
Keyvan AMINI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chitosan; Cyclosporine; administration & dosage; pharmacology; Immunohistochemistry; Nerve Regeneration; drug effects; Rats; Sciatic Nerve; chemistry; injuries
- From: Chinese Journal of Traumatology 2014;17(1):12-18
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo assess the effect of cyclosporine A (CsA) loaded in chitosan conduit on bridging the sciatic nerve defects in a rat model.
METHODSA 10 mm sciatic nerve defect was bridged using a chitosan conduit filled with 10 μl carrier-drug dilution (10 μg/L CsA). In control group, the conduit was filled with the same volume of carrier dilution alone. The regene-rated fibers were studied 4, 8 and 12 weeks after surgery.
RESULTSThe functional study confirmed faster recovery of the regenerated axons in treatment group than control group (P<0.05). There was statistically significant difference of the gastrocnemius muscle weight ratios between treatment and control groups (P<0.05). Morphometric indices of regenerated fibers showed that the number and diameter of the myelinated fibers in CsA-treated animals were significantly higher than those in control group. In immunohistochemistry, the location of reactions to S-100 in CsA group was clearly more positive than control group.
CONCLUSIONCsA loaded in a chitosan conduit results in improvement of functional recovery and quantitative morphometric indices of sciatic nerve. It is easily available without any complications compared with its systemic administration.
