Protective Effect of Hyaluronic Acid on Cyclophosphamide-Induced Cystitis in Rats.
10.4111/kju.2009.50.8.797
- Author:
Jung Won LEE
1
;
Youn Wha KIM
;
Jung Sik HUH
;
Sun Ju LEE
Author Information
1. Department of Urology, School of Medicine, Kyung Hee University, Seoul, Korea. sjlee@khu.ac.kr
- Publication Type:Original Article
- Keywords:
Hyaluronic acid;
Cyclophosphamide;
Cystitis
- MeSH:
Animals;
Cyclophosphamide;
Cystitis;
Female;
Humans;
Hyaluronic Acid;
Rats;
Rats, Sprague-Dawley;
Urinary Bladder;
Urodynamics
- From:Korean Journal of Urology
2009;50(8):797-804
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We investigated the effect and duration of hyaluronic acid (HA) on the bladder of rats with cyclophosphamide (CYP)-induced cystitis. MATERIALS AND METHODS: CYP (250 mg/kg) was injected intraperitoneally in female Sprague-Dawley rats to induce cystitis. HA (0.5%) was instilled intravesically. First, the rats were separated into 2 groups for urodynamic study (CYP group, CYP+HA group), and we assessed the inter-contraction interval, maximal voiding pressure (MVP), and pressure threshold of the 2 groups. Second, the rats were separated into 3 groups to assess the duration of effect of HA (CYP group; HA-1 group: HA was instilled 1 day before injection of CYP; HA-3 group: HA was instilled 3 days before injection of CYP), and we assessed the voiding interval for 7 days after confining the rats in metabolic cages. RESULTS: The inter-contraction interval of the CYP+HA group (236.0+/-18.3 min) was significantly longer than that of the CYP group (178.0+/-13.4 min; p<0.03), but the MVPs of both groups did not differ significantly (32.3+/-1.2 vs. 34.0+/-2.4 cmH2O, respectively; p=0.56). The pressure threshold of the CYP+HA group (9.9+/-0.5 cmH2O) was significantly longer than that of the CYP group (13.2+/-0.7 cmH2O; p=0.002). The voiding interval decreased in each of the 3 groups according to the time after cystitis had been induced. The voiding interval was maximally decreased on the second day and returned to the pre-CYP injection level on the fifth day in each group. There was a significant difference in voiding interval among the 3 groups on the second day (p<0.05). There was no significant difference between the CYP+HA-1 and CYP+HA-3 groups on the second day (p>0.05). CONCLUSIONS: HA has an effect of increasing the voiding interval in rats with CYP-induced cystitis, and the duration of effect is concluded to be 5 days.
