The influence of rhAng-1 on endothelial junctions of BBB after focal cerebral ischemia/reperfusion in rats.
- Author:
Hang YU
1
;
Qing-Bo JIA
;
Yi-Xue XUE
Author Information
- Publication Type:Journal Article
- MeSH: Angiopoietin-1; pharmacology; Animals; Antigens, CD; metabolism; Blood-Brain Barrier; cytology; metabolism; Brain Ischemia; metabolism; Cadherins; metabolism; Endothelium, Vascular; cytology; drug effects; Humans; Male; Occludin; metabolism; Rats; Rats, Wistar; Recombinant Proteins; pharmacology; Reperfusion Injury; metabolism; Zonula Occludens-1 Protein; metabolism
- From: Chinese Journal of Applied Physiology 2013;29(2):147-152
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis study was performed to determine whether recombinant human angiopoietin-1 (rhAng-1) decreases the permeability of the blood-brain barrier (BBB) in focal cerebral ischemia and reperfusion rats, whether RhAng-1 opens the BBB by affecting tight junction associated proteins zonnula occludin-1 (ZO-1), occludin and adherens junction protein vascular endothelial (VE)-cadherin.
METHODSThe rats were divided into eight groups randomly( n = 10): (1) sham-operated group; (2) ischemia group; (3)-(5) ischemia/reperfusion (middle cerebral artery occlusion and reperfusion (MCAO/R) 12 h, 48 h, and 7 days) and 0.9% saline groups; (6)-(8) ischemia/reperfusion (MCAO/R 12 h, 48 h, and 7 days) and rhAng-1 groups. The Bee permeability was assessed by Evans blue extravasation. The messenger RNA and protein expressions of ZO-1, occludin, and VE-cadherin were determined by reverse transcription-polymerase chain reaction, Western blot, and immunohistochemistry assays.
RESULTSThe BBB permeability and the brain infarct volume were significantly decreased after rhAng-1 injection. The expressions of ZO-1, occludin, and VE-cadherin were increased after rhAng-1 injection.
CONCLUSIONrhAng-1 may decrease the permeability of BBB in MCAO/R rats by upregulation of ZO-1, occludin, and VE-cadherin.
