Effects of microencapsulated CHO cells modified with maspin gene on the motility and adhesiveness of breast carcinoma cells Bcap37.
- Author:
Yue-long PAN
1
;
Shu ZHENG
;
Jia-ping PENG
;
Qi DONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Breast Neoplasms; pathology; CHO Cells; Capsules; Cell Adhesion; Cell Movement; Coculture Techniques; Cricetinae; Cricetulus; Female; Glycoproteins; biosynthesis; genetics; Humans; Hyaluronan Receptors; biosynthesis; genetics; Serpins; genetics; Tumor Cells, Cultured
- From: Chinese Journal of Oncology 2005;27(6):342-346
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of microencapsulated Chinese hamster ovary (CHO) cells modified with maspin gene on the motility and adhesiveness of breast carcinoma cells Bcap37 and to explore the possibility and feasibility of its clinical application in treatment of malignant tumors.
METHODSAfter the Bcap37 cells were co-cultured with the microencapsulated CHO cells modified with maspin gene, their motility and adhesion to vascular endothelial cells (ECV304), changes in CD44v6 and E-cadherin expression were examined.
RESULTSAfter the treatment, the motility of Bcap37 cells, their adhesion to vascular endothelial cells ECV304 and the CD44v6 expression were significantly reduced. The adhesiveness of Bcap37 cells and their E-cadherin expression were significantly enhanced.
CONCLUSIONThe microencapsulated CHO cells modified with maspin gene decrease motility and adhesiveness of breast carcinoma cells Bcap37, which help explain the anti-metastatic effects of maspin.
