Screening and identification of phage-displayed polypeptides specifically binding to human gastric cancer with high metastatic potential to peritoneum.
- Author:
Ke-dong ZHANG
1
;
Xin-ning GUO
;
Li YANG
;
Dong-tao ZHANG
;
Fei-hu BAI
;
Hai-ping JIANG
;
Hui-hong ZHAI
;
Yong-zhan NIE
;
Kai-chun WU
;
Dai-ming FAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Binding Sites; Cell Line, Tumor; Enzyme-Linked Immunosorbent Assay; Female; Humans; Male; Mice; Mice, Inbred BALB C; Peptide Library; Peptides; metabolism; Peritoneal Neoplasms; secondary; Protein Array Analysis; methods; Protein Binding; Sensitivity and Specificity; Stomach Neoplasms; metabolism; pathology
- From: Chinese Journal of Oncology 2005;27(7):397-400
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEBy means of phage-display technique, to screen polypeptides that specifically bind to human gastric cancer with high metastatic potential to peritoneum.
METHODSTwo human gastric cancer cell lines were used: GC9811-P with high metastatic potential to peritoneum and its wild type parental GC9811, to carry out subtractive screening with a phage display-12 peptide library.
RESULTSAfter three rounds of screening, 40 phage clones bond to GC9811-P cells were randomly selected. When injected into the peritoneal cavity of nude mice, 6 of the 40 clones did not bind to mouse peritoneum as examined by immunohistochemical staining. They were considered to be capable of binding specifically to GC9811-P cells. Sequence analysis revealed two different exogenous peptides: TLNINRLILPRT and SMSI(X)SPYI(XXX).
CONCLUSIONTwo peptides have been obtained that specifically bind to a gastric cancer cell variant GC9811-P, which easily disseminates to the peritoneum. Whether or not they could block GC9811-P metastasis to peritoneum in vivo remains to be determined.
