Mechanisms of capsaicin-induced apoptosis of human melanoma A375-S2 cells.
- Author:
Xian-feng GONG
1
;
Min-wei WANG
;
Takashi IKEJIMA
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; pharmacology; Apoptosis; drug effects; Apoptosis Regulatory Proteins; biosynthesis; Capsaicin; pharmacology; Caspases; metabolism; Cell Line, Tumor; Dose-Response Relationship, Drug; Humans; Melanoma; pathology; Signal Transduction; Skin Neoplasms; pathology
- From: Chinese Journal of Oncology 2005;27(7):401-403
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the mechanisms of capsaicin-induced apoptosis of human melanoma A375-S2 cells.
METHODSMTT assay, fluorescence microscopy, DNA agarose gel electrophoresis, flow cytometry and Western blot analysis were carried out to assess the morphological and biochemical changes of A375-S2 cells after capsaicin treatment.
RESULTSCapsaicin induced A375-S2 cell death in a time- and dose-dependent manner. Sub-diploid peak was seen at 24 h after 250 micromol/L capsaicin treatment, and apoptotic bodies and DNA ladder were observed at 36 h after capsaicin treatment. The expression of inhibitor of caspase activated DNase (ICAD) was reduced with the lapse of time.
CONCLUSIONCapsaicin induces A375-S2 cell apoptosis and down-regulation of ICAD contributes to this process.
