Flow cytometry-pathology combined study of breast cancer.
- Author:
Zhi-yong YU
1
;
Jin-ming YU
;
Tai-huang WU
;
Pei-ying ZHUO
;
Sheng-fang WANG
;
Mei LI
;
Xia ZHANG
;
Bao-yu LÜ
;
Yan-bing LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aneuploidy; Breast Neoplasms; metabolism; pathology; Carcinoma, Ductal, Breast; metabolism; pathology; DNA, Neoplasm; analysis; Female; Flow Cytometry; Humans; Middle Aged; Mutation; Prognosis; Proto-Oncogene Proteins c-bcl-2; biosynthesis; genetics; Receptors, Estrogen; biosynthesis; genetics; S Phase; Tumor Suppressor Protein p53; biosynthesis; genetics
- From: Chinese Journal of Oncology 2005;27(7):420-422
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the relation between histopathologic grading and some of the cytogenetic and molecular biology characteristics of breast cancer.
METHODSOn the basis of estrogen receptor (ER) expression, DNA content, S-phase fraction (SPF), bcl-2 and mutant p53 protein (mtp53) expression were examined by FCM in 121 breast cancer patients. In 66 patients with invasive ductal breast cancer, histopathologic grading was also examined.
RESULTSThe aneuploidy rate and DNA index (DI) were significantly different in grade I, II and III breast cancer. SPF and mtp53 expression significantly increased with increase in histopathologic grading (P < 0.05), but bcl-2 did not show this trend. SPF and mtp53 expression were significantly more in breast cancer with negative ER than in those with positive ER (P < 0.05). Again, no such differences in bcl-2 regardless of ER expression. Correlations existed between DI vs SPF, DI vs mtp53, and SPF vs mtp53 expressions (P < 0.01) but bcl-2 did not correlate with any one of them.
CONCLUSIONCytogenetic and molecular biology studies on the basis of histopathologic grading may provide more information in prognostic prediction of breast cancer.
