Establishment of dendritomas by fusion of human dendritic cells with human hepatocellular carcinoma cell line HLE cells.
- Author:
Xin GUAN
1
;
Ji-run PENG
;
Xi-sheng LENG
Author Information
- Publication Type:Journal Article
- MeSH: Cancer Vaccines; biosynthesis; Carcinoma, Hepatocellular; pathology; Cell Fusion; methods; Cell Line, Tumor; Cells, Cultured; Dendritic Cells; cytology; Humans; Hybrid Cells; Liver Neoplasms; pathology
- From: Chinese Journal of Oncology 2005;27(8):465-467
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct dendritomas by fusion of human dendritic cells with HLE cells, a human hepatocellular carcinoma cell line.
METHODSHLE cells were cultured in RPMI 1640 with 15% FCS. Human dendritic cells (DCs) were obtained from peripheral blood monocytes cultured in the presence of GM-CSF and IL-4 for 7 days, matured with TNF-alpha and PGE(2) for 2 days. The DCs and HLE cells were labeled with green fluorescence dye PKH67-GL and red fluorescence dye PKH26-GL, respectively, and fused in 50% polyethylene glycol (PEG) + 10% dimethyl sulfoxide (DMSO) to generate dendritomas for rapid fluorescence-activated cell sorting (FACS).
RESULTSDendritomas with dual red-green fluorescence were constructed successfully, and FACS analysis showed the effective fusion rate was 16.8%.
CONCLUSIONWith fluorescence dyes PKH67-GL and PKH26-GL as fusion markers, dendritomas for rapid fluorescence-activated cell sorting are constructed, which may throw new light on immunotherapy of hepatocellular carcinoma.
