Detection of methylation of hMSH2 gene promoter region of esophageal cancer.
- Author:
Gong-yuan ZHANG
1
;
Chun-xiao MA
;
Qiu-liang LIU
;
Xiao-ping LE
;
Yi DING
;
Qin-xian ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Base Pair Mismatch; Carcinoma, Squamous Cell; genetics; pathology; DNA Methylation; Esophageal Neoplasms; genetics; pathology; Female; Gene Expression Regulation, Neoplastic; Humans; Male; MutS Homolog 2 Protein; genetics; Promoter Regions, Genetic; Transfection
- From: Chinese Journal of Oncology 2005;27(9):541-543
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect methylation in promoter region of hMSH2 gene in esophageal cancer.
METHODSSpecimens of cancer and normal tissues freshly removed from 32 cases of esophageal cancer patients without previous radiotherapy, chemotherapy or other treatment were preserved at -80 degrees C within 30 min. Methylation specific PCR (MSP) was used to detect methylation of mismatch repair gene (MMR) hMSH2 in promoter region in esophageal cancer and normal esophageal tissues.
RESULTSThe frequencies of methylation of hMSH2 gene in promoter region of cancer and normal esophageal tissues were 32.4% (11/32) and 0/30 (0%), respectively, and significant difference was found between the two groups (P < 0.01). The frequency of methylation in elder patients (> or = 70 years old) was significantly higher than that in younger patients (< 70 years old) (P < 0.05). Methylation was less frequently found in grade I-II (18.2%) than in grade III-IV (70.0%) (P < 0.05).
CONCLUSIONMethylation of hMSH2 gene in promoter region is related to patients' age and histopathological grade of the esophageal cancer.
