Expression of IgVH and B7-1 in proteome of the human colorectal carcinoma cell lines.
- Author:
Ying-tao ZHANG
1
;
Yi-ping GENG
;
Bao-chang LAI
;
Lü-sheng SI
;
Yi-li WANG
Author Information
- Publication Type:Journal Article
- MeSH: B7-1 Antigen; biosynthesis; genetics; Cell Line, Transformed; Colorectal Neoplasms; genetics; immunology; metabolism; Humans; Immunoglobulin Heavy Chains; biosynthesis; genetics; Immunoglobulin Variable Region; biosynthesis; genetics; Neoplasm Proteins; biosynthesis; genetics; immunology; Peptide Mapping; Proteome; isolation & purification
- From: Chinese Journal of Oncology 2005;27(11):648-652
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo conduct a proteomic analysis of human colorectal carcinoma cell lines LS174T and SW480 by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).
METHODSThe total proteins of human colorectal carcinoma cell lines LS174T and SW480 were separated with 2-DE using immobilized pH gradient strips and analyzed by MALDI-TOF-MS to obtain peptide mass fingerprints (PMFs). Proteins were identified by using Mascot software to search protein databases.
RESULTSGood resolution 2-DE maps were obtained. Some proteins including immunoglobulin heavy chain variable region (IgVH) and co-stimulatory molecule B7-1 were identified. IgVH and B7-1 were confirmed by electrospray ionization tandem spectrometry (ESI-MS/MS) and immunocytochemistry.
CONCLUSIONThere are IgVH and B7-1 expressions in human colorectal carcinoma cell lines LS174T and SW480. Results obtained will help to elucidate the mechanisms of tumor immune escape.
