Regulatory role of Syk gene on vascular endothelial growth factor C expression in breast cancer.
- Author:
Xian-liang CHEN
1
;
Liang LI
;
Ya-jie ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Breast Neoplasms; genetics; metabolism; pathology; Carcinoma, Ductal, Breast; genetics; metabolism; pathology; Carcinoma, Lobular; genetics; metabolism; pathology; Carcinoma, Medullary; genetics; metabolism; pathology; Cell Line, Tumor; Female; Genetic Vectors; Humans; Intracellular Signaling Peptides and Proteins; genetics; metabolism; physiology; Lymphatic Metastasis; Middle Aged; NF-kappa B; metabolism; Plasmids; Protein-Tyrosine Kinases; genetics; metabolism; physiology; RNA, Messenger; metabolism; Syk Kinase; Transfection; Vascular Endothelial Growth Factor C; genetics; metabolism
- From: Chinese Journal of Pathology 2011;40(12):805-809
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Syk on the VEGF-C expression in breast cancer.
METHODSImmunohistochemical EnVision method was used to detect the protein expression of Syk, NFκB and VEGF-C in breast carcinoma; and the relationship between protein expression of Syk, NFκB, VEGF-C and lymph node metastasis was analysed. MDA-MB-231 cells were transfected with pcDNA3.1(-)-Syk, and the effect of Syk gene on the VEGF-C and NFκB expression was determined.
RESULTSIn the lymph node metastatic group, a lower expression rate of Syk and higher expression rate of VEGF-C and NFκB were detected as compared to the non-metastatic group. The expression of Syk was negatively associated with NFκB (r = -0.448, P = 0.002) and VEGF-C (r = -0.620, P = 0.000) expression, and VEGF-C was associated with the nuclear expression of NFκB (r = 0.310, P = 0.036). Compared with the non-transfected cells, the pcDNA3.1(-)-Syk transfected MDA-MB-231 cells showed significantly lower transcriptional level of VEGF-C mRNA, expression level of VEGF-C protein and NFκB activity (P < 0.05).
CONCLUSIONSSyk may play an important role in the lymph node metastasis of breast cancer. It may down-regulate the expression of VEGF-C by inhibiting the activity of NFκB, which thus suppresses lymph node metastasis of breast cancer.
