Expression of enoyl CoA hydratase 1 reduces cell proliferation and migration in mouse hepatocarcinoma cells.
- Author:
Jun ZHANG
1
;
Jian-wu TANG
;
Mei-ying SONG
;
Bo WANG
;
Rong-kuan LI
;
Li HOU
;
Yu-hong HUANG
;
Mei WANG
;
Bo SONG
;
Yan-Ling JIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Cycle; Cell Line, Tumor; Cell Movement; Cell Proliferation; Cytoplasm; enzymology; Down-Regulation; Enoyl-CoA Hydratase; genetics; metabolism; Gene Expression Regulation, Neoplastic; Liver Neoplasms, Experimental; enzymology; pathology; Lymphatic Metastasis; Mice; Plasmids; RNA, Small Interfering; genetics; Transfection
- From: Chinese Journal of Pathology 2011;40(12):830-833
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of enoyl CoA hydratase 1 (ECH1) and the effect when down-regulation of ECH1 gene expression in mouse hepatocarcinoma cell.
METHODSImmunofluorescence was used for detecting the expression of ECH1, and stably transfected Hca-F cells with pGPU6/GFP/Neo-shRNA-ECH1 expression plasmids. Cell proliferation was assessed by Cell counting kit-8 (CCK8) assay. The Boyden-transwell assay (8 µm pore size) was performed to analyze the inhibitory effect of shRNA on Hca-F cell migration and invasion.
RESULTSECH1 expression was obtained in the cytoplasm and upregulated expression in Hca-F cells than that in Hca-P cells. The down-regulation of ECH1 could inhibit the cell proliferation of Hca-F cells, decrease the number of cell pass through Transwell (27.07 ± 17.49) compared with scramble-negative (72.38 ± 18.83) and Hca-F controls (59.06 ± 30.33), decrease the migration capacities of Hca-F cells, increase the ratio of Hca-F cells in S phase (86.1%) compared with scramble-negative (75.8%) and Hca-F controls (66.2%) and decrease the ratio of G(1) phase (9.4%) compared with scramble-negative (24.2%) and Hca-F controls (30.3%).
CONCLUSIONECH1 serves as a potential critical factor attributes to tumor lymphatic metastasis.
