The photodynamic therapy on Streptococcus mutans biofilms using erythrosine and dental halogen curing unit.
- Author:
Young-Ho LEE
1
;
Ho-Won PARK
;
Ju-Hyun LEE
;
Hyun-Woo SEO
;
Si-Young LEE
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Load; drug effects; Bacteriological Techniques; Biofilms; drug effects; Curing Lights, Dental; classification; Erythrosine; therapeutic use; Humans; Microbial Viability; drug effects; Microscopy, Confocal; Photochemotherapy; methods; Photosensitizing Agents; therapeutic use; Sonication; Streptococcus mutans; drug effects; Sucrose; pharmacology; Time Factors
- From: International Journal of Oral Science 2012;4(4):196-201
- CountryChina
- Language:English
- Abstract: The purpose of our study was to evaluate the effect of photodynamic therapy (PDT), using erythrosine as a photosensitizing agent and a dental halogen curing unit as a light source, on Streptococcus mutans in a biofilm phase. The S. mutans biofilms were formed in a 24-well cell culture cluster. Test groups consisted of biofilms divided into four groups: group 1: no photosensitizer or light irradiation treatment (control group); group 2: photosensitizer treatment alone; group 3: light irradiation alone; group 4: photosensitizer treatment and light irradiation. After treatments, the numbers of colony-forming unit (CFU) were counted and samples were examined by confocal laser scanning fluorescence microscopy (CLSM). Only group 4 (combined treatment) resulted in significant increases in cell death, with rates of 75% and 55% after 8 h of incubation, and 74% and 42% at 12 h, for biofilms formed in brain-heart infusion (BHI) broth supplemented with 0% or 0.1% sucrose, respectively. Therefore, PDT of S. mutans biofilms using a combination of erythrosine and a dental halogen curing unit, both widely used in dental clinics, resulted in a significant increase in cell death. The PDT effects are decreased in biofilms that form in the presence of sucrose.
