The growth of Staphylococcus aureus and Escherichia coli in low-direct current electric fields.

Dunya ZITUNI; Heidi SCHÜTT-GEROWITT; Marion KOPP; Martin KRÖNKE; Klaus ADDICKS; Christian HOFFMANN; Martin HELLMICH; Franz FABER; Wilhelm NIEDERMEIER

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The growth of Staphylococcus aureus and Escherichia coli in low-direct current electric fields.

Author: Dunya ZITUNI ¹ ; Heidi SCHÜTT-GEROWITT ² ; Marion KOPP ¹ ; Martin KRÖNKE ² ; Klaus ADDICKS ³ ; Christian HOFFMANN ³ ; Martin HELLMICH ⁴ ; Franz FABER ⁵ ; Wilhelm NIEDERMEIER ¹
Author Information

1. Department of Prosthetic Dentistry, School of Dental and Oral Medicine, Cologne University, Cologne, Germany.
2. Institute for Medical Microbiology, Immunology and Hygiene, Cologne University, Cologne, Germany.
3. Institute for Anatomy I, Cologne University, Cologne, Germany.
4. Institute for Medical Statistics, Informatics and Epidemiology, Cologne University, Cologne, Germany.
5. Department of Pre-Clinical Dentistry, School of Dental and Oral Medicine, Cologne University, Cologne, Germany.
Publication Type:Journal Article
MeSH: Bacterial Load; radiation effects; Bacteriological Techniques; Caseins; Cell Wall; radiation effects; Culture Media; Cytoplasm; radiation effects; Electricity; Escherichia coli; growth & development; radiation effects; Gels; Humans; Microbial Viability; radiation effects; Microscopy, Electron, Transmission; Protein Hydrolysates; Sodium Chloride; Staphylococcus aureus; growth & development; radiation effects; Water
From: International Journal of Oral Science 2014;6(1):7-14
CountryChina
Language:English
Abstract: Electrical potentials up to 800 mV can be observed between different metallic dental restorations. These potentials produce fields in the mouth that may interfere with microbial communities. The present study focuses on the impact of different electric field strengths (EFS) on the growth of Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922) in vitro. Cultures of S. aureus and E. coli in fluid and gel medium were exposed to different EFS. Effects were determined by calculation of viable counts and measurement of inhibition zones. In gel medium, anodic inhibition zones for S. aureus were larger than those for E. coli at all field strength levels. In fluid medium, the maximum decrease in the viable count of S. aureus cells was at 10 V⋅m(-1). Field-treated S. aureus cells presented ruptured cell walls and disintegrated cytoplasm. Conclusively, S. aureus is more sensitive to increasing electric field strength than E. coli.