Bacterial entombment by intratubular mineralization following orthograde mineral trioxide aggregate obturation: a scanning electron microscopy study.

Jun Sang Yoo; Seok-woo Chang; So Ram OH; Hiran Perinpanayagam; Sang-min Lim; Yeon-jee Yoo; Yeo-rok OH; Sang-bin Woo; Seung-hyun Han; Qiang Zhu; Kee-yeon Kum

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Bacterial entombment by intratubular mineralization following orthograde mineral trioxide aggregate obturation: a scanning electron microscopy study.

Author: Jun Sang YOO ¹ ; Seok-Woo CHANG ² ; So Ram OH ³ ; Hiran PERINPANAYAGAM ⁴ ; Sang-Min LIM ⁵ ; Yeon-Jee YOO ³ ; Yeo-Rok OH ¹ ; Sang-Bin WOO ¹ ; Seung-Hyun HAN ⁶ ; Qiang ZHU ⁷ ; Kee-Yeon KUM ³
Author Information

1. Department of Dentistry, U Dental Hospital, Seoul, Korea.
2. Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, Korea.
3. Department of Conservative Dentistry, Dental Research Institute, Seoul National University Dental Hospital, Seoul National University School of Dentistry, Seoul, Korea.
4. Division of Restorative Dentistry, Schulich School of Medicine & Dentistry, University of Western Ontario, London, Canada.
5. Department of Conservative Dentistry, Jukjeon Dental Hospital, College of Dentistry, Dankook University, Jukjeon, Korea.
6. Department of Oral Microbiology & Immunology, Dental Research Institute and BK21 Program, Seoul National University School of Dentistry, Seoul, Korea.
7. Division of Endodontology, Department of Oral Health and Diagnostic Sciences, School of Dental Medicine, University of Connecticut Health Center, Farmington, USA.
Publication Type:Journal Article
MeSH: Aluminum Compounds; therapeutic use; Calcification, Physiologic; physiology; Calcium Compounds; therapeutic use; Crystallization; Dental Pulp Cavity; microbiology; Dentin; microbiology; Drug Combinations; Enterococcus faecalis; ultrastructure; Humans; Methylmethacrylates; therapeutic use; Microscopy, Electron, Scanning; Oxides; therapeutic use; Root Canal Filling Materials; therapeutic use; Root Canal Obturation; methods; Root Canal Preparation; instrumentation; Silicates; therapeutic use; Time Factors; Zinc Oxide-Eugenol Cement; therapeutic use
From: International Journal of Oral Science 2014;6(4):227-232
CountryChina
Language:English
Abstract: The time domain entombment of bacteria by intratubular mineralization following orthograde canal obturation with mineral trioxide aggregate (MTA) was studied by scanning electron microscopy (SEM). Single-rooted human premolars (n=60) were instrumented to an apical size #50/0.06 using ProFile and treated as follows: Group 1 (n=10) was filled with phosphate buffered saline (PBS); Group 2 (n=10) was incubated with Enterococcus faecalis for 3 weeks, and then filled with PBS; Group 3 (n=20) was obturated orthograde with a paste of OrthoMTA (BioMTA, Seoul, Korea) and PBS; and Group 4 (n=20) was incubated with E. faecalis for 3 weeks and then obturated with OrthoMTA-PBS paste. Following their treatments, the coronal openings were sealed with PBS-soaked cotton and intermediate restorative material (IRM), and the roots were then stored in PBS for 1, 2, 4, 8 or 16 weeks. After each incubation period, the roots were split and their dentin/MTA interfaces examined in both longitudinal and horizontal directions by SEM. There appeared to be an increase in intratubular mineralization over time in the OrthoMTA-filled roots (Groups 3 and 4). Furthermore, there was a gradual entombment of bacteria within the dentinal tubules in the E. faecalis inoculated MTA-filled roots (Group 4). Therefore, the orthograde obturation of root canals with OrthoMTA mixed with PBS may create a favorable environment for bacterial entombment by intratubular mineralization.