OBJECTIVEThe suspension-cultured protocorms of Dendrobium candidum were transplanted on the solid culture medium for studying the factors influencing their differentiation and growth.

METHODThe growth and differentiation of protocorms were detected when different base-media, plant phytohormones, carbohydrates and pH were applied.

RESULT AND CONCLUSIONThe diluted MS medium promotes the growth of protocorms but inhibit differentiation. The phytohormone NAA, IAA, IBA and GA are helpful to the growth and differentiation of protocorms, however, the low pH values and presence of autoclaved fungi have suppressive effects on protocorms. The fungi also induce morphological variation of protocorms.