Isolation and purification of total tubeimosides from Bolbostemma paniculatum by macroporous resin.
- Author:
Ting-jun MA
1
;
Peng-fei TU
;
Fei-jie LV
Author Information
- Publication Type:Journal Article
- MeSH: Cucurbitaceae; chemistry; Plants, Medicinal; chemistry; Resins, Synthetic; Rhizome; chemistry; Saponins; isolation & purification; Technology, Pharmaceutical; methods; Triterpenes; isolation & purification
- From: China Journal of Chinese Materia Medica 2005;30(10):744-746
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo select the optimum purification process of all tubeimosides from Bolbostemma paniculatum by macroporous resin.
METHODStatic absorption test and deabsorption test were carried out to screen the best macroporous resin for all tubeimosides. The single factor test, orthogonal experimental designs and variance analysis were applied to optimize the manipulation parameters of macroporous resin.
RESULTThe macroporous resin NKA possessed the strongest absorption ability to all tubeimosides among the 9 resins studied, in addition to an easy de-absorption property. The optimum absorption conditions were A2B3C1, namely concentration of feed 30 mg x mL(-1), diameter: height of the chromatography column 1:7, the weight ratio of raw material and resins 1:2.
CONCLUSIONThe above experimental results can provide experimental basis for large-scale purification process of all tubeimosides.
