Study on tissue culture and plant regeneration of Nervilia fordii.
- Author:
Qin DU
1
;
Wen-Li CHEN
;
Zhen-Hua WANG
Author Information
- Publication Type:Journal Article
- MeSH: Carbon; pharmacology; Culture Media; Orchidaceae; growth & development; Plant Growth Regulators; pharmacology; Plants, Medicinal; growth & development; Regeneration; Rhizome; growth & development; Tissue Culture Techniques; methods
- From: China Journal of Chinese Materia Medica 2005;30(11):812-814
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETissue culture of Nervilia fordii to get its regeneration.
METHODEffects on indusement of rhizoma and plant regeneration of different implants, density of hormones, additives were studied.
RESULTThe best implant was conn. Effect of 6-BA 2 mg x L(-1) were better than 6-BA 1 on rhizoma reducing. The coconut juice and active carbon could increase the growth of rhizoma.
CONCLUSIONBud could be induced on 1/2MS + 6-BA 2 mg x L(-1) by inoculating corm on culture mediem, and could grow lots of rhizoma after inoculating on the culture mediem containing 10% coconut juice and 1 per thousand active carbon. The white rhizoma could be induced to corms and regeneration plants on 1/2MS + 1 per thousand active carbon. The green rhizoma could be induced directerly to regeneration plants on 1/2MS + 6-BA 2 + NAA 2.
