Purification and characterization of the proliferation of rat osteoblast-like cells UMR-106 from pilose antler.
- Author:
Dong-Yun LIN
1
;
Xiao-Nan HUANG
;
Li-Jing KE
;
Xiao-Chao CHEN
;
Xiu-Yun YE
;
Yu-Shu HUO
;
Ping-Fan RAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antlers; chemistry; Bone Neoplasms; pathology; Cell Line, Tumor; Cell Proliferation; drug effects; Deer; Insulin-Like Growth Factor I; secretion; Materia Medica; isolation & purification; pharmacology; Osteoblasts; metabolism; pathology; Osteosarcoma; pathology; Rats; Serum Albumin; isolation & purification; pharmacology
- From: China Journal of Chinese Materia Medica 2005;30(11):851-855
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe activity of deer serum albumin on proliferation of rat osteogenic-like cells UMR-106 and the IGF-I secretion were investigated in order to elucidate pilose antler's bone-strengthening mechanism.
METHODDeer serum albumin was isolated from freeze-dry pilose antler powder extract. The methods were Sephacryl S-200HR gel filtration, POROS 20QE ion-exchange and TSK G3000SW chromatographies. The effect of deer serum albumin on proliferatio of UMR-106 cells was assaied by MTT, and the secretion of IGF-I of UMR-106 cells was assaied by RIA.
RESULTDeer serum albumin, with the molecular weight of 56.3 kDa, significantly increased the proliferation rate of the osteoblast-like UMR-106 cell and IGF-I secretion. When concentration of deer serum albumin reached 0.149 microg x mL(-1), UMR-106 cell proliferation rate was 241.03% and IGF-I secretion was 66.89 ng x mL(-1).
CONCLUSIONThe concentration of deer serum albumin, from 14.9 ng x mL(-1) to 14.90 microg x mL(-1), significantly increased the proliferation rate of the osteoblast-like UMR-106 cell and IGF- I secretion.
