Effects of mineral cations on the accumulation of tanshinone II A and protocatechuic aldehyde in the adventitious root culture of Salvia niltiorrhiza.
- Author:
Xiao-Hong GUO
1
;
Wen-Yuan GAO
;
Hai-Xia CHEN
;
Lu-Qi HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Benzaldehydes; metabolism; Catechols; metabolism; Copper; pharmacology; Diterpenes, Abietane; Iron; pharmacology; Magnesium; pharmacology; Manganese; pharmacology; Phenanthrenes; metabolism; Plant Roots; growth & development; metabolism; Plants, Medicinal; growth & development; metabolism; Salvia miltiorrhiza; growth & development; metabolism; Tissue Culture Techniques; Zinc; pharmacology
- From: China Journal of Chinese Materia Medica 2005;30(12):885-888
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of mineral cations on the growth of Salvia niltiorrhiza adventitious roots and the accumulation of tanshinone II A and protocatechuic aldehyde.
METHODThe adventitious roots were cultured under different concentrations of metal cations and the contents of tanshinone II A and protocatechuic aldehyde were determined by HPLC.
RESULTFe2 + and Mn2+ in MS medium are suitable for adventitious root growth, but Cu2+ and Mg2+ in high concentration could improve root proliferation, and Zn2+ has no obvious effect on root growth. As for tanshinone II A and protocatechuic aldehyde biosynthesis, Cu2+ and Zn2+ would inhibit the biosynthesis of protocatechuic aldehyde, furthermore, proper Fe2+ and Mg2+ concentration could advance the biosynthesis of protocatechuic aldehyde, whereas, Mn2+ in high concentration could accelerate the biosynthesis of protocatechuic aldehyde. Cu2+, Zn2+, Mg2+ in low concentration and Fe2+ and Mn2+ in high concentration would all advance the biosynthesis of tanshinone II A.
CONCLUSIONThe mineral cations have obvious effects on the secondary metabolites biosynthesis in adventitious root culture of S. niltiorrhiza.
