The protective effect of cholic acid on an in vitro injury model of ischemia-reperfusion in cerebral microvascular endothelial cells.
- Author:
Yong-Hong GAO
1
;
Zheng-Zhong YUAN
;
Fu-Ling NIU
;
Wei CUI
;
Ling-Qun ZHU
;
Shuo-Ren WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; blood supply; Brain Ischemia; pathology; Cell Survival; drug effects; Cells, Cultured; Cholic Acid; pharmacology; Endothelial Cells; drug effects; pathology; Male; Microcirculation; Neuroprotective Agents; pharmacology; Rats; Rats, Sprague-Dawley; Reperfusion Injury; pathology
- From: China Journal of Chinese Materia Medica 2005;30(12):930-932
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an in vitro injury model of ischemia-reperfusion in cerebral microvascular endothelial cells of rats and observe the protective effect of cholic acid.
METHODCultured rat microvascular endothelial cells were subjected to the oxygen-glucose deprivation (OGD) (Krebs solution) and recovery of oxygen-glucose, which simulated in vitro ischemia and reperfusion injury, and treated with cholic acid. The A value was measured with MIT chromatometry.
RESULTCultured cells were impaired after OGD for 4 hours and recovery of oxygen-glucose for 12 hours, the A value of the cells treated with cholic acid was significantly higher than that of the cells without treatment (P < 0.01).
CONCLUSIONCholic acid could obviously protect rat cerebral microvascular endothelial cells from injury induced by an in vitro ischemia-reperfusion.
