Cell suspension culture and flavonoids production in Saussurea involucrate.
- Author:
Li-qin WU
1
;
Shun-xing GUO
;
Pei-gen XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Carbon; Cell Proliferation; drug effects; Culture Media; Flavonoids; biosynthesis; Hydrogen-Ion Concentration; Plant Growth Regulators; pharmacology; Plants, Medicinal; cytology; growth & development; metabolism; Saussurea; cytology; growth & development; metabolism; Seeds; cytology; growth & development; metabolism; Sucrose; Tissue Culture Techniques; methods
- From: China Journal of Chinese Materia Medica 2005;30(13):965-968
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a system of cell suspension culture for Saussurea involucrate.
METHODThe effects of different factors on cell growth and flavonoids production of S. involucrate were systematically studied including the media, initial pH values of the medium, carbon sources, inoculum quantity, and plant growth substance.
RESULTThe optimum medium was N6, initial pH values of the medium was 5.8, sucrose concentration was 50 g x L(-1), inoculum quantity was 60-80 g x L(-1) FW. Medium supplemented with BA (0.5 mg x L(-1)) and NAA (3 mg x L(-1)) was suitable for cell growth, but medium containing BA (0.2 mg x L(-1)) and NAA (2 mg x L(-1)) was suitable for flavonoids production.
CONCLUSIONCell growth and flavonoids production in the suspention of S. involucrate culture cell should be optimized.
