Determination of cholesteryl palmitate in Oviductus Ranae by HPLC.
- Author:
Yong-sheng WANG
1
;
Da-cheng JIANG
;
Qin MENG
;
En-si WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cholesterol Esters; analysis; Chromatography, High Pressure Liquid; methods; Female; Materia Medica; analysis; chemistry; Oviducts; chemistry; Quality Control; Rana temporaria
- From: China Journal of Chinese Materia Medica 2005;30(13):990-991
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a new method for the determination of cholesteryl palmitate in Oviductus Ranae.
METHODA HPLC method was set up, using Zorbax Silica column and cyclohexane-diethyl ether (40:1) as mobile phase with a flow rate of 1.0 mL x min(-1), and the UV detection wavelength was 203 nm.
RESULTThe calibration curve was linear over the range of 0.60-8.92 microg (r = 0.9997), the average recovery of the method was 98.4%. RSD 1.8% (n = 6).
CONCLUSIONThe results showed that method was reliable and accurate.
