Effects of ginkgolides on gene expression of hepatic cytochrome P-450 in rats.
- Author:
Xiu-fen YANG
1
;
Nai-ping WANG
;
Fan-dian ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aryl Hydrocarbon Hydroxylases; biosynthesis; genetics; Cytochrome P-450 CYP1A1; biosynthesis; genetics; Cytochrome P-450 CYP1A2; biosynthesis; genetics; Cytochrome P-450 CYP2B1; biosynthesis; genetics; Cytochrome P-450 CYP3A; Cytochrome P-450 Enzyme System; biosynthesis; genetics; Cytochrome P450 Family 4; Gene Expression Regulation; Ginkgo biloba; chemistry; Ginkgolides; isolation & purification; pharmacology; Liver; metabolism; Male; Plants, Medicinal; chemistry; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2005;30(13):1009-1013
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of ginkgolides on gene expression of hepatic cytochrome P-450 in rats.
METHODSprague-Dawley rats were administered ginkgolides (100 mg x kg(-1) body weight) through oral gavage once daily for four consecutive days. The level of gene expression in liver tissues was analyzed by competitive reverse transcription-polymerase chain reaction (competitive RT-PCR).
RESULTA single and prospective band of CYP1A1, CYP1A2, CYP2B1/B2, CYP2C11, CYP2E1, CYP4A1 and cyclophilin was observed after polymerase chain reaction (PCR) when the reactive system of reverse transcription (RT) had no target RNA, which confirmed the competitor had a specific capacity to bind to the CYP or cyclophilin primer. CYP1A1 mRNA was not dectectable in the livers of untreated control rats and ginkgolides-treated rats. The levels of CYP2C11 and CYP2E1 were not changed by ginkgolides treatment. In contrast, the levels of gene expression for CYP1A2 and CYP2B1/B2 were decreased, however, the levels of gene expression for CYP3A1 and CYP4A1 in ginkgolides group were distinctly increased compared with the control.
CONCLUSIONA specific effect of ginkgolides on cytochrome P-450 gene expression was observed in this investigation. Ginkgolides had various effects on different cytochrome P-450 isoforms.
