Preparation and quality control of low molecular weight chitosan.
- Author:
Xuan HUANG
1
;
Fu-Qiang HU
;
Hong YUAN
;
Xiao-Ying YING
Author Information
- Publication Type:Journal Article
- MeSH: Cellulase; metabolism; Chitosan; chemistry; Chromatography, Gel; Colorimetry; Hydrolysis; Molecular Weight; Quality Control; Technology, Pharmaceutical; methods
- From: China Journal of Chinese Materia Medica 2005;30(14):1076-1079
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare the low molecular weight chitosan (LMWC) and establish the method for quality control.
METHODUse enzymatic degradation to prepare LMWC with chitosan, and separate by ultrafiltration; the molecular weight and purity were determined by gel permeation chromatography (GPC) and colorimetry respectively.
RESULTLMWC was prepared by control the hours of enzymatic degradation and ultrafiltered through filter with cutoff molecular of 10K Dalton and 50 K dalton; the average molecular weight was 20 K dalton and the purity was (96.60 +/- 1.56)%.
CONCLUSIONThe condition of enzymatic degradation is geniality and easy to control, LMWCs with different molecular weight can separate by ultrafiltration efficiently; the quality of LMWC can control with gel permeation chromatography (GPC) and colorimetry.
