Study on extraction of coumarins in Cnidium monieri by supercritical CO2 and separation of compositions analysis.
- Author:
Hong MI
1
;
Li-Li QU
;
Yu-Lin REN
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, Supercritical Fluid; Cnidium; chemistry; Coumarins; chemistry; isolation & purification; Fruit; chemistry; Furocoumarins; chemistry; isolation & purification; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2005;30(14):1080-1082
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the chemical constituents of supercritical carbon dioxide extraction products from Cnidium monieri.
METHODFour-factor and three-level orthogonal experimental design was used to optimize the SFE conditions as guided by the content of total coumarins in the extract. The chemical constituents were separated and identified by recrystalization.
RESULTOptimum extraction process was established: 25 MPa as extraction pressure, 50 degrees C as extraction temperature, 6.5 MPa as separation pressure and 60 degrees C as separation temperature.
CONCLUSIONChanges in extraction pressure, temperature, time, pulverized degree and separation pressure affect the extracting results remarkably. The two kinds of chemical constituents were separated by recrystallization from C. monieri and identified by the methods of UV, IR, MS, NMR.
