Effects of Alopecia Areata Serum on Proliferation of Cultured Dermal Papilla Cells.
- Author:
Sang Jun LEE
;
Yang Hoon CHO
;
Woo Young SIM
;
Choong Rim HAW
- Publication Type:Original Article
- Keywords:
Dermal papilla cells;
Alopecia areata serum
- MeSH:
Alopecia Areata*;
Alopecia*;
Cell Count;
Cell Proliferation;
Epithelial Cells;
Fibroblasts;
Hair;
Humans;
Scalp
- From:Korean Journal of Dermatology
1998;36(6):1032-1036
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Dermal papilla cells, which are mesenchymal components of the hair bulb are considered to play an important role in the regulation of hair growth by production of diffusible factors that stimulate follicular epithelial cells. Degenerative changes in the dermal papilla cells in the involved scalp of alopecia areata cases indicate that these cells are one of the important targets in this disease. OBJECTIVE: We investigated the effects of serum from alopecia areata patients on the proliferation of dermal papilla cells. METHOD: Dermal papilla cells and fibroblasts from normal human scalp were cultured in DMEM media with 10% or 20% of normal and alopecia areata serum for 48hrs and 96hrs. Cell proliferation was measured by cell counts and [3H]-thymidine incorpoartion. RESULTS: Both 10% and 20% alopecia areata serum had no significant effects on the proliferation of dermal papilla cells and fibroblasts after 48hrs and 96hrs. CONCLUSION: These results suggest that there are no serum factors that inhibit the proliferation of dermal papilla cells.
