Expression of phosphorylated NMDA receptor 1 in the cerebral cortex after NMDA microinjection in vivo.
- Author:
Ming-Yan HEI
1
;
Mi-Li XIAO
;
Shou-Jin KUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cerebral Cortex; drug effects; metabolism; Female; Fluorescent Antibody Technique; Hypoxia-Ischemia, Brain; metabolism; Male; Microinjections; N-Methylaspartate; administration & dosage; Phosphorylation; Rats; Rats, Sprague-Dawley; Receptors, N-Methyl-D-Aspartate; analysis; drug effects
- From: Chinese Journal of Contemporary Pediatrics 2007;9(1):51-53
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe activation of N-methyl-D-aspartate(NMDA) receptors plays critical roles in the pathogenesis of diseases of the brain. This study aimed to examine the expression of phosphor-NR1 S897 in the cerebral cortex after NMDA microinjection in vivo.
METHODSForty seven-day-old Sprague-Dawley rats were randomly assigned into normal control and NMDA injection groups. The rats from the NMDA injection group were injected with 10 mmol of NMDA and were sacrificed 1 hr after injection. 2, 3, 5-triphenyltetrazolium chloride (TTC) and fluorescent immunohistochemical stainings were conducted and the fluorescence intensity OD value between the two groups was compared.
RESULTSTTC staining from the two groups was normal. Expression of phosphor-NR1 S897 in the cerebral cortex of the ipsilateral hemisphere to injection in the NMDA injection group decreased significantly compared with the normal control group, with OD values of 0.366 +/- 0.087 vs 1.364 +/- 0.268 (P < 0.01).
CONCLUSIONSNMDA microinjection, as a hypoxia-ischemia (HI) insult, significantly decreased the expression of phosphor-NR1 S897. This indicates the importance of the "HI-NMDA-phospho-NR1 S897 dephosphorylation-cell damage" pathway in HI brain damage.
