Preparation of carboxymethyl-chitosan with different molecular weight and its effects on proliferation of skin fibroblasts and keratinocytes.
- Author:
Zheng WANG
1
;
Wanshun LIU
;
Baoqin HAN
;
Ruyong YAO
;
Changzheng WEI
Author Information
1. Institute of Oceanology,The Chinese Academy of Sciences, Qingdao 266071, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Proliferation;
drug effects;
Cells, Cultured;
Chitosan;
analogs & derivatives;
chemical synthesis;
pharmacology;
Fibroblasts;
cytology;
drug effects;
Humans;
Keratinocytes;
cytology;
drug effects;
Mice;
Molecular Weight;
Skin;
cytology
- From:
Journal of Biomedical Engineering
2007;24(2):340-344
- CountryChina
- Language:Chinese
-
Abstract:
Effects of carboxymethyl-chitosan (CM-Chitosan) with different molecular weight on the proliferation of skin fibroblasts and keratinocytes were examined in vitro; bFGF and EGF, as controls, were seperately used for comparison. Chitosan with different molecular weight was prepared by acid degradation and oxidation degradation; CM-Chitosan with different molecular weight was synthesized from corresponding Chitosan. Microscopy and MTT method were applied to evaluate the different effects. The results demonstrated that CM-Chitosan with different molecular weight promoted the proliferation of skin fibroblasts and keratinocytes at 1-1000 ppm, and the concentration at 100 ppm had the strongest effects. The effects of low molecular weight CM-Chitosan were greater than those of high molecular weight CM-Chitosan. CM-Chitosan (Mn= 3KD) had the strongest promotive effects on skin fibroblasts and keratinocytes; it had equivalent effects when compared with bFGF and EGF.
