BMP-2 gene carried by biodegradable scaffold and fibrinous gel for repairing segmental radial defect in rabbit.
- Author:
Jianjun LI
1
;
Enbo WANG
;
Hongbin SUN
;
Dong HAN
;
Huan WANG
;
Lunhao BAI
;
Lei LI
;
Xueyong LIU
;
Xinxiang XU
Author Information
1. Department of Orthopaedics, The Second Hospital of China Medical University, Shenyang 110004, China. lijianjun71@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Absorbable Implants;
Adenoviridae;
genetics;
metabolism;
Animals;
Biocompatible Materials;
Bone Morphogenetic Protein 2;
biosynthesis;
genetics;
Bone Substitutes;
Caprolactam;
analogs & derivatives;
chemistry;
Fracture Healing;
Gels;
Genetic Therapy;
Humans;
Implants, Experimental;
Lactic Acid;
chemistry;
Polyesters;
Polymers;
chemistry;
Rabbits;
Radius Fractures;
therapy;
Tissue Engineering;
Tissue Scaffolds
- From:
Journal of Biomedical Engineering
2007;24(2):345-349
- CountryChina
- Language:Chinese
-
Abstract:
Adenovirus carrying BMP-2 gene, after being mixed with fibrinous gel, was siphoned off on biodegradable scaffolds (PLA/PCL). The composite was used to repair 1.5 cm long radius defect in rabbits. Four methods were in use in the experiments: Ad-BMP-2 plus fibrinous gel and PLA/PCL (Group A), reconstructed hBMP-2 plus fibrinous gel and PLA/PCL (Group B), Ad-Lacz plus fibrinous gel and PLA/PCL (Group C), and fibrinous gel and PLA/PCL (Group D). Results showed that the defects treated in Group A were repaired with much more new bone regenerated, bridged earlier and stronger than those in Group B 12 weeks after operation. The defects treated in the other two groups could not attain osseous tissue healing. BMP-2 gene carried by biodegradable scaffold and fibrinous gel is easy to conduct and has very strong osteoinduction ability. It is really a good method to repair segmental bone defects.
