The killing effect of focused ultrsound activating protoporphyrin IX on S180 cells.
- Author:
Yaohui REN
1
;
Xiaobing WANG
;
Pan WANG
;
Qiao HAO
;
Quanhong LIU
Author Information
1. College of Life Science, Shanxi Normal University, Xi'an 710062, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
drug effects;
radiation effects;
Mice;
Mice, Inbred ICR;
Photochemotherapy;
methods;
Photosensitizing Agents;
pharmacology;
Protoporphyrins;
pharmacology;
Sarcoma 180;
pathology;
therapy;
Sonication;
Tumor Cells, Cultured;
Ultrasonics
- From:
Journal of Biomedical Engineering
2007;24(3):626-630
- CountryChina
- Language:Chinese
-
Abstract:
The killing effect on S180 cells was studied using the combination of protoporphyrin IX and focused ultrasound at the frequency of 2.2 MHz and different intensities. Cell viability was determined by trypan blue exclusion test, morphology changes were evaluated by means of scanning electron microscopy and transmission electron microscopy after ultrasonic exposure. The results indicated that protoporphyrin IX(PPIX) alone showed no significant effect on S180 cells when compared with that of control group. Ultrasound alone and ultrasound combined with PPIX groups showed some anti-tumor effect, which became more noticeable as the ultrasound intensity and PPIX concentration increased, and when the concentration of PPIX increased to 120 microM, the ultrasound combined with PPIX exerted a more significant anti-tumor effect than did the ultrasound alone in the same experiment.
