Effect of human silicotic alveolar macrophages on the expression of the collagen type I in human embryonic lung fibroblasts.
- Author:
Xiao-hui HAO
1
;
Xian-hua WANG
;
Li ZHANG
;
Fang YANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Cells, Cultured; Collagen Type I; metabolism; Fibroblasts; metabolism; Humans; Lung; metabolism; Macrophages, Alveolar; immunology; Male; Silicosis; immunology
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(2):69-72
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of the cultured supernatant of human silicotic alveolar macrophages (AM) on the expression of the collagen type I in human embryonic lung fibroblasts.
METHODSHuman alveolar macrophages were collected from a silicotic patient by bronchoalveolar lavage and exposed to silicon dioxide for 18 h. Then the cultured supernatant were used to culture human embryonic lung fibroblasts for 6 h, 12 h, 18 h, 24 h, 36 h, 48 h, 72 h. Then detected collagen anabolism and secretion with (3)H-proline detected the expression of the procollagen type I in the fibroblast with immunological method detected the quantity of collagen Type I in FB supernatant with Western blot.
RESULTSThe anabolism and secretion of collagen were increased in cultured supernatant of silicotic AM exposed to SiO(2), Along with the time, the expression of collagen type I increased. In cultured supernatant of silicotic AM exposed to SiO(2), ((3)H-proline: 1096.500 +/- 76.400, 707.000 +/- 62.160, OD: 0.314 +/- 0.011, OD: 14.218 +/- 0.342.
CONCLUSIONSiO(2) may affect the expression of collagen through AM mediation and participate in the formation of lung fibrosis.
