Genetic effects of workers occupationally exposed to mercury using three genetic end-points.
- Author:
Wei-jun GUO
1
;
Xiao-ying ZHAO
;
Su-ying LI
;
Chun-hua CHEN
;
Zhi-jian CHEN
;
Jian-lin LOU
;
Wei ZHENG
;
Hong-ping DENG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Case-Control Studies; Chemical Industry; Comet Assay; Female; Humans; Male; Mercury; Micronucleus Tests; Middle Aged; Mutation Rate; Occupational Exposure; adverse effects; Young Adult
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(2):84-86
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigating genetic effects of workers occupationally exposed to mercury (Hg).
METHODSThe peripheral lymphocytes from 20 workers exposed to mercury and 20 controls were measured with micronucleus test, comet assay, hrpt gene mutation test and TCR gene mutation test.
RESULTSThe mean micronuclei rate(MNR) and mean micronucleated cells rate(MCR) in 20 workers were (5.90 +/- 0.91) per thousand and (5.30 +/- 0.81) per thousand, respectively while MNR and MCR in controls were (1.50 +/- 0.47) per thousand and (1.30 +/- 0.31) per thousand respectively, The difference of MNR and MCR between workers and controls was very significant (P < 0.01). The mean tail length (MTL) of workers and controls were (3.16 +/- 0.31) and (0.99 +/- 0.07) microm, respectively. The mean tail moment (MTM) of workers and controls were 1.63 +/- 0.22 and 0.39 +/- 0.03, respectively, There was a significant difference in MTL and MTM between workers and controls(P < 0.01). When the average mutation frequencies (Mfs-hprt) of hprt and (Mfs-TCR) of TCR of workers were compared with those of controls, there were not significant difference (P > 0.05).
CONCLUSIONThe results of the investigation indicated that the adverse genetic effects in workers occupationally exposed to mercury could be detected.
