Involvement of Caspase-3 and p38 mitogen-activated protein kinase in MMT-induced apoptosis in PC-3M cells.
- Author:
Li-xiang WANG
1
;
Ji-ping ZENG
;
Wen QIN
;
Wei-wen CHEN
;
Ya-lun ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; physiology; Caspase 3; metabolism; Caspase Inhibitors; Cell Line; Humans; Imidazoles; pharmacology; Male; Organometallic Compounds; toxicity; Phosphorylation; Prostate; cytology; Pyridines; pharmacology; p38 Mitogen-Activated Protein Kinases; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(3):152-154
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the function of Caspase-3 and p38 MAPK in MMT-induced apoptosis in PC-3M cells.
METHODSAfter incubation of PC-3M cells with 1 mmol/L MMT, the activity of Caspase-3 was examined. The influence on cells viability of Z-DEVD-FMK, a Caspase-3-specific peptide inhibitor, was also examined. Western blot was used to examine the change of p38 MAPK. The effect on cells viability and Caspase-3 activity of SB203580, a specific inhibitor of p38 MAPK, were also examined.
RESULTSThe activity of Caspase-3 increased significantly in MMT-induced apoptosis in PC-3M cells /9P < 0.01), and Z-DEVD-FMK could protect cells from apoptosis (P < 0.01). In this course, the phosphorylation of p38 MAPK could be observed. SB203580 inhibited Caspase-3 activity (P < 0.05) and prevented PC-3M cells from MMT-induced apoptosis (P < 0.05).
CONCLUSIONCaspase-3 and p38 MAPK are involved in MMT-induced PC-3M cells apoptosis.
