Cadherin switching induced by P120-catenin can promote the migration and invasion of oral squamous cell cancer cells.
- VernacularTitle:P120-连环蛋白介导钙黏蛋白转换促进口腔鳞状细胞癌细胞迁移和侵袭的实验研究
- Author:
Zhong CHEN
1
;
Mei ZHANG
2
;
Yong XU
3
Author Information
- Publication Type:Journal Article
- Keywords: P120-catenin; cadherin switching; oral squamous cell cancer
- MeSH: Cadherins; Carcinoma, Squamous Cell; Catenins; Cell Line, Tumor; Cell Movement; Humans; Mouth Neoplasms; Neoplasm Invasiveness; Neoplasm Metastasis; Transfection
- From: West China Journal of Stomatology 2017;35(2):183-186
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe main goal is to investigate the role of P120-catenin (P120ctn) in cadherin switching, as well as migration and invasion, of oral squamous cell cancer (OSCC) cells.
METHODSThe plasmid pGFP-V-RS-P120ctn shRNA was used to transfect TSCCA cells and significantly reduce the expression of P120ctn in these cells. Real-time fluorescent quantitative polymerase chain reaction and Western blot were conducted to determine the mRNA and protein expression levels of P120ctn, E-cadherin (E-cad), and N-cadherin (N-cad). By contrast, the Transwell cell invasion and cell migration assay was used to determine the invasion and migration capacities before and after the transfection.
RESULTSAfter the plasmid pGFP-V-RS-P120ctn shRNA was transfected into the TSCCA cells, we found that as the P120ctn expression significantly decreased, E-cad mRNA and protein expression decreased significantly. Moreover, N-cad mRNA and protein expression increased significantly (P<0.05). Lastly, the cell migration and invasion capacities were augmented significantly (P<0.05).
CONCLUSIONSIn OSCC cells, P120ctn may be involved in cadherin switching and promote metastasis and invasion.
