Role of CCL5/CCR5 in the perineural invasion of salivary adenoid cystic carcinoma cells.

Tianyi LI; Zhiyuan Shen

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Role of CCL5/CCR5 in the perineural invasion of salivary adenoid cystic carcinoma cells.

Author: Tianyi LI ¹ ; Zhiyuan SHEN ²
Author Information

1. Dept. of Pediatric Dentistry, Stomatological Hospital of Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, China.
2. Dept. of Oral and Maxillofacial Surgery, Stomatological Hospital of
Publication Type:Journal Article
Keywords: CCL5; CCR5; perineural invasion; salivary adenoid cystic carcinoma; salivary glands
From: West China Journal of Stomatology 2017;35(5):479-483
CountryChina
Language:Chinese
Abstract:
OBJECTIVEThis study aimed the role of the CCL5/CCR5 axis in the perineural invasion of salivary adenoid cystic carcinoma (SACC) cells.
METHODSImmunohistochemical analysis and flow cytometric analysis were conducted to detect the expression of the chemokine receptor CCR5 in SACC cells. Enzyme linked immunosorbent assay (ELISA) was performed to determine the expression of CCL5 in the supernate of human nerve cells. The flow cytometric analysis was applied to observe the changes in F-actin in SACC-LM cells, which were pretreated with CCL5. To assess the effects of the CCL5/CCR5 axis on the migration and invasion of SACC-LM cells, we performed a scratch test and invasion assay under CCL5 stimulation.
RESULTSCCR5 was highly expressed in SACC cells. The concentration of CCL5 in the supernatant of human nerve cells was (359.2±15.8), (696.4±22.6) pg·mL⁻¹. The CCL5/CCR5 axis promoted the migration and invasion of SACC-LM cells.
CONCLUSIONSThe CCL5/CCR5 axis may be involved in the perineural invasion of SACC cells.