Effect of Helicobacter pylori-encoded CagA on biological behavior of gastric adenocarcinoma cells in vitro.
- Author:
Xin SONG
1
;
Hui-Xin CHEN
;
Jie CHEN
;
Ai-Ping BAI
;
Xiao-Yan LI
;
Wei CHEN
;
Min-Hu CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; microbiology; pathology; ultrastructure; Antigens, Bacterial; genetics; Bacterial Proteins; genetics; Cell Line, Tumor; Cell Movement; Extracellular Space; Helicobacter pylori; genetics; pathogenicity; Humans; Intercellular Junctions; ultrastructure; Mutation; Stomach Neoplasms; microbiology; pathology; ultrastructure
- From: Chinese Journal of Oncology 2008;30(5):339-342
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Helicobacter pylori-encoded CagA on biological behavior of gastric adenocarcinoma AGS cells.
METHODSWith experiment-control system of the wild-type CagA positive strain and isogenic CagA negative mutant strain of Helicobacter pyroli (Hp) were used as control and experimental groups, respectively. The cell contact, migration and invasion were examined by light and electron microscopy and invasion assay.
RESULTSThe AGS cells infected by Hp strain with positive wild-type CagA showed more severely changed tight junction, wider intercellular space, loss of cell contacts, and higher migrating and invasive ability.
CONCLUSIONHp CagA may lead to loss of cell contacting and higher migrating and invading ability of gastic cells, and accelerates the malignant progress of tumor.
