Overexpression of human tumor metastasis-related gene TMSG-1 suppresses cell proliferation and invasion of a highly metastatic prostate cancer cell line PC-3M-1E8 in vitro.
- Author:
Jing SU
1
;
Jiang-feng YOU
;
Jie-liang WANG
;
Xiang-lin CUI
;
Wei-gang FANG
;
Jie ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Cell Movement; Cell Proliferation; Gene Expression Regulation, Neoplastic; Genes, Tumor Suppressor; Humans; Male; Membrane Proteins; genetics; metabolism; Mice; NIH 3T3 Cells; Neoplasm Invasiveness; Prostatic Neoplasms; metabolism; pathology; RNA, Messenger; metabolism; Sphingosine N-Acyltransferase; Transfection; Tumor Suppressor Proteins; genetics; metabolism
- From: Chinese Journal of Oncology 2008;30(6):404-407
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of tumor metastasis-related gene TMSG-1 overexpression on the proliferation and invasion of a highly metastatic prostate cancer cell line in vitro.
METHODSThe eukaryotic expression plasmids containing full-length TMSG-1 cDNAs were stably transfected into the highly metastatic prostate cancer cell line PC-3M-1E8. Clones highly expressing TMSG-1 were identified by RT-PCR and Western Blot analysis after G418 screening. The cell proliferation was detected by cell growth curve, MTT assay and soft agar colony formation assay. The invasive potential of tumor cells in vitro was tested by Matrigel invasion assay.
RESULTSThree TMSG-1 overexpression clones were selected. Cell growth curve and MTT assay showed that TMSG-1 overexpression clones exhibited a strong inhibition of proliferation compared with that of the parental cells or those transfected with vector alone from the third day of culture (P <0.05). In vitro analysis also showed that the TMSG-1 transfected clones exhibited a decreased clonogenicity in soft agar compared with that of the parental cells or those transfected with vector only (P < 0.05). TMSG-1 expression significantly suppressed cell invasion in vitro of TMSG-1-transfected PC-3M-IE8 cells (P < 0.05).
CONCLUSIONThe TMSG-1 protein may serve as a tumor metastasis suppressor due to inhibiting cell proliferation and invasion of the highly metastatic prostate cancer cell line PC-3M-1E8.
